After 10 episodes beginning in April 2007, it took a few months off, returning in August at the later time of 9:25 pm allowed the show to move from a PG to an M rating, and to include more adult-oriented humour.

This segued into the opening credits, during which musical director Cameron Bruce (formerly of GUD) and house band "The Bearded Ladies" played the show's theme song.

This was generally followed by a musical act, which featured guests including Evermore (Pilot episode), Thirsty Merc, Sneaky Sound System, Sarah Blasko, Kisschasy, The Cat Empire, Kate Miller-Heidke, Dappled Cities Fly, Expatriate, Kid Confucious, Something for Kate, Dog Trumpet, Tim Rogers, Clare Bowditch, Watussi, Colin Hay, Operator Please, The Hands, Butterfingers and Jimmy Barnes.

After this monologue, he introduced the stand-up guest of the evening, among whom were Denise Scott, Tom Gleeson, Dave Hughes, Wil Anderson, Tommy Dean, Eddie Ifft, Josh Thomas, Frank Woodley, Ed Byrne, Kitty Flanagan, Danny Bhoy, Tom Rhodes, Arj Barker, Justin Hamilton, Gary Eck, Fiona O'Loughlin, Charlie Pickering and Greg Fleet.

Occasionally, these were musical guests, such as an interview with Colin Hay from Australian band Men at Work, or a performance from Dein Perry and the Tap Dogs.

Comedian Flacco had a regular segment on the show, often in the role of "Private Dick" in a stand-up routine that parodies the format and visual style of film noir and pulp novels.

The segment originated as a "20 questions" interview but gradually drifted away from this format, with Hooper moving into humorous story-telling—often relating to embarrassing events in her own life—and comedy routines.

The show also regularly featured short pre-recorded video sketches that divided up the live-to-tape studio segments: New Moods in Intelligent Design This sketch featured a voiceover from McDermott with accompanying text on screen explaining "Why God Didn't Design" some form of absurd or dysfunctional fictional animal such as the Pyjamadillo (a pyjama-wearing nocturnal armadillo).

This segment lightheartedly satirized the major arguments for intelligent design as opposed to natural selection, by suggesting that the animal in question doesn't exist because God knew better than to create it (rather than its inability to evolve or survive because of the creature's impracticality).

The Company of Strangers Presents This sketch, written by Flacco's alter-ego Paul Livingston, was typically a short animation with a cleverly punned subtitle.