Ludwig's wife, Mary, asked him to set Martin Luther's translations of psalms xii, xiii, xxxviii and lxxxvi, which he did between 1524 and 1526.
One personal letter of Stoltzer's is still extant, dated February 23, 1526 and addressed to Albert, Duke of Prussia in Königsberg; in this letter Stoltzer relates the news of a recently completed psalm setting and intimates that he would like to join Albrecht's court.
Stoltzer's extant works amount to some 150 pieces, gathered in 30 publications and 60 manuscripts.
His early motets often make use of numerological signifiers of religious importance; later works show influence from the Netherlands school of composers, such as imitation and the use of multiple choirs.
He composed four masses, as well as fourteen introits spanning the church year from Christmas to Easter.