Not much is known about his early life, but he begins appearing in various Antwerp archives of around 1530 working as a calligrapher as well as an instrumentalist: trumpet, flute and tenor pipe are listed as instruments that he owned.
Soon afterwards, Susato was joined by Petrus Phalesius the Elder in Leuven and Christopher Plantin, also in Antwerp, and the Low Countries became a regional center of music publishing.
It is possible that Susato also ran a musical instrument business, and he attempted several times to form partnerships with other publishers but none was successful.
Most important of his publications in terms of distribution and influence were the Souterliedekens of Clemens non Papa, which were metrical psalm settings in Dutch, using the tunes of popular songs.
He produced one book of dance music in 1551, Het derde musyck boexken ... alderhande danserye, composed of pieces in simple but artistic arrangement.