He is the identical twin brother of NFL Hall of Fame former Tampa Bay Buccaneers cornerback and safety Ronde Barber.

[3] Barber rushed for almost 1,400 yards in his junior season, helping lead the Cavaliers to a share of the ACC title.

He began his career with a mediocre rookie campaign, in which he started at running back and rushed for 511 yards and three touchdowns.

In 1998, he was replaced as a starter and went through a disappointing, injury-riddled year before rebounding in 1999, when he accumulated 1,639 all-purpose yards, utilizing his punt return and receiving skills.

Barber and the Giants represented the NFC in Super Bowl XXXV in 2001, but lost the game to the Baltimore Ravens.

In the last regular season game of 2002, Tiki exploded for 203 yards against the Philadelphia Eagles, at that time one of the league's best defenses.

His performance helped the Giants clinch a playoff berth, but the team fell to the San Francisco 49ers in the wild-card round.

Poignantly, Barber broke the record on the final offensive play of the game, when he scored a game-winning touchdown to end an otherwise dismal 6–10 season for the Giants on a high note.

The Giants promptly shut out the Washington Redskins 36-0 two days after Wellington Mara was buried, proper homage to their beloved owner, to whom Tiki himself was especially close.

During week 14 at Carolina, Barber rushed for 112 yards to help the Giants end a four-game losing skid.

In his final game with the Giants, a playoff loss to the Philadelphia Eagles, Barber rushed for 137 yards on 26 carries.

"[8] Barber has stated a tackle from Eagles middle linebacker Jeremiah Trotter during the 2006 season confirmed his decision to retire.

In the very next NFL season following Barber's retirement, the New York Giants went on to defeat the previously undefeated 18–0 New England Patriots to win Super Bowl XLII in one of the greatest upsets in sports history.

When head coach Tom Coughlin arrived, he taught Barber to hold the ball vertically instead of horizontally, a practice taken from rugby football.

Barber began to focus on strength training and lifting in 2004 upon the suggestion of his new running backs coach, Jerald Ingram.

He refined upon his patience during game-play, which is needed in football to wait for blocks to set and formed holes to emerge in the offensive line before accelerating forward.

[20] Barber additionally benefited from an improved offensive line and Coughlin's emphasis on the Giants' new strategic running game.

Heavy sports media attention was placed on the comment; Tiki apologized for it shortly afterwards, saying he only meant to convey the Giants' performance was 'unacceptable.'

Barber originally began his broadcasting career on WFAN radio and the WCBS local news in New York.

[27] In January 2022,[28] Barber became a radio co-host for WFAN with Brandon Tierney, on weekday afternoons airing 10-2 pm (EST).

[29] In June 2023, the New York Post broke the news that Barber would take the spot of Craig Carton on afternoon drive at WFAN with Evan Roberts beginning in July 2023.

His character was filmed dressing down an unseen private and used by KITT to distract the owner of the illegal club long enough to allow for capture by Michael.

Barber made his Broadway debut as Don in Kinky Boots January 21, 2019, and played a limited run until March 3.

"[33] Barber is pursuing business and investment opportunities, reportedly inking a contract to serve as a partner and investor in O Beverages LLC, the maker of fruit-infused bottled water[34] as well as opening a high-tech cycling gym in NYC.

[35] Barber is co-chairman and co-founder of Thuzio, which provides an online marketplace for athletes to be booked for local speaking appearances, coaching, and personal events.

[41][42] Ronde was a cornerback who played for the Tampa Bay Buccaneers, winning Super Bowl XXXVII against the Oakland Raiders, and was elected to the NFL Hall of Fame in 2023.

[47] On April 5, 2010, while Cha was seven months pregnant with their twin daughters, it was announced that Barber and his wife were separating after 11 years of marriage.

[48][49] It later became public that Barber had been cheating on Cha with 23-year-old Traci Lynn Johnson, whom he met while she was an NBC intern and he worked on the Today show.

[54] Just eight days after his divorce from Cha was final, on July 20, 2012, Barber and Johnson married in a simple civil ceremony in a New York courtroom.

[58] In 2014, Barber supported CC Sabathia's charity, the PitCCh In Foundation, by running in the 2014 New York City Marathon.