In veterinary medicine, tiludronic acid is used to treat navicular disease and bone spavin in horses.

Non-nitrogenous bisphosphonates are metabolized by osteoclasts to compounds that then replace a portion of the adenosine triphosphate (ATP) molecule, making it non-functional.

It has specifically been shown to improve lameness in horses with osteoarthritis of the distal hock joints (bone spavin)[5] and vertebral column.

Although RLP is thought to have certain benefits, including decreased cost and reduced risk of adverse effects, some diseases must be treated systemically, such as osteoarthritis of the vertebral column.

[8] The effects of regional limb perfusion has come into question due to in vitro studies showing that high doses given by RLP or intraarticular injection may damage articular cartilage by chondrocyte apoptosis.