It was used to carry payloads for the United States Air Force (USAF), NASA and National Oceanic and Atmospheric Administration (NOAA).

The first launch occurred on 5 September 1988, carrying a classified payload for the U.S. National Reconnaissance Office.

The engines were refurbished, and the rockets' guidance and control systems were upgraded by Delco Electronics.

A fourteenth rocket, G-10, based on Titan II B-108, but incorporating an oxygen tank from B-80, was not launched and is preserved at the Evergreen Aviation & Space Museum in McMinnville, Oregon.

The remaining 42 Titan II missiles were stored at Davis-Monthan AFB with most being broken up for salvage.