The Toklat River (Lower Tanana: Tootl'o Huno') is an 85-mile (137 km) tributary of the Kantishna River in central Alaska in the United States[3] It drains an area on the north slope of the Alaska Range on the south edge of the Tanana Valley southwest of Fairbanks.

[4] It issues from unnamed glaciers in the northern Alaska Range in Denali National Park and Preserve, northeast of Denali.

[4] It flows generally northwest through hilly country[4] to the tundra to the north of the Alaska Range.

The river was described as the Toclat by Lt. H.T.

Other names or variants include Tootl'ot Huno, Tootl'ot Huno' Hutl'ot, Tootl'ot No'  and Tutlut River.