The genus contains the following species:[2] Viruses in Tombusvirus are non-enveloped, with icosahedral and spherical geometries, and T=3 symmetry.

Genomes are linear and non-segmented, positive sense, single-stranded RNA, around 4-5.4kb in length.

[1] These virions have a regular surface structure and are composed of 17% nucleic acid.

The virus exits the host cell by tubule-guided viral movement.

[1] The virus uses the cis-regulatory elements, Tombus virus defective interfering (DI) RNA region 3 and Tombusvirus 5' UTR to control expression of defective interfering RNAs and viral RNA replication.