Stroot was born in Nordhorn, Germany to a Dutch mother and German father.

[1] He studied physiotherapy and worked with disabled people before becoming a coach.

[2] In 2013, Stroot was appointed manager of German side SV Meppen.

In 2016, he was appointed manager of Dutch side FC Twente.

In 2021, he was appointed manager of German side VfL Wolfsburg, replacing Stephan Lerch.