Service began in 2007 with a route between the City Hall (Municipalidad) and a market place at Centra Sur (Southern Transfer Station).

Buses run down the middle of the street, are separated from other traffic, and stop at stations approximately every kilometre.

Security is provided by Guatemala City's transit police, with officers in fluorescent yellow vests deployed at most stations on the route.

In January 1999, mayor Fritz García Gallont proposed implementing the Transmetro BRT project.

However, it would not be until January 2004 when then mayor Álvaro Arzú confirmed that he had considered the Transmetro as a solution to alleviate congestion in the city.

The opening date was pushed to February 2007 when phase one, named "Eje Sur" (South line), began revenue service in the city.

This second phase coincided with the revitalization project of 6th Avenue in the historic downtown, and received support from private sector developers.

The "Eje Nor-oriente" line began operation in 2014 to serve Zone 18 from the Atlantida neighbourhood and the Downtown core.

Passengers must pay the fare each time they enter the Transmetro, which means a two-way trip costs Q2.00, regardless of how far one goes.

In November 2015, payments by the SIGA smart card were no longer accepted because of a multitude of problems and disagreements.

The gates received public support due to the COVID-19 pandemic, which discouraged many from the use of cash payments to reduce the risk of transmission.

Line 12 starts from Zone 1 in Guatemala City, it runs through the Civic Centre, Bolivar Avenue, Trebol Station, Raul Aguilar Batres Way, and finally terminating at Centra Sur in Zone 12 of neighbouring Villa Nueva city.

The change in bus types was done to allow the line to travel through the historic centre as the streets are narrower compared to those of the CA1.

This line, as mentioned earlier, runs through the historic downtown, this means that during its journey it will pass by popular landmarks of the city such as the Torre del Reformador, the Acueducto de Pinula, the Bank of Guatemala, Civic Centre, and 6th Avenue, a popular pedestrian-only section of the city.

The main goal of this line was to make the historic centre more accessible through safe and reliable public transportation.

Line 2 also has an express service to provide direct connections between Zone 1, and the UMG university campus.

In place of the express service, San Jose de la Montaña station was opened near the campus.

Departing from Plaza Barrios-FEGUA Station at 18th Street in Zone 1, ti travels through very important, high traffic areas and sites of interest like Colon Park.

This line began operating on April 25, 2014, and continues to be under construction specially in the northern segment of the route to improve road conditions and exclusive traffic lane.

Additionally, FEGUA and Plaza Barrios were no longer noted as interchange stations, despite a walking connection remaining available to Line 12.

[14] The is intended to cover more suburban service, as well as provide more direct access to the USAC university campus.

[15] This line was slated to use a different rolling stock from the standard Marcopolo BRT, instead using a set of city-style buses which were to be 100% electric.

[15] This line would use a different rolling stock from the standard Marcopolo BRT, instead using a set of city-style buses which are 100% electric.

Since the announcement of TuBus operations by the city, this previously proposed line and its alignment will now be used for the route traveling between Centra Atlantida and Lomas del Norte in Zone 17.

[18][20] Line 21 started as a pilot project to measure sustainability, and conduct a user needs assessment for service to the USAC university campus,[21] in the southern end of the city.