The Truce of Nice, signed on June 18, 1538, ended hostilities, leaving Turin in French hands but effecting no significant change in the map of Italy.

The war strengthened animosity between the Habsburgs and the French, and reinforced ties between France and the Ottoman Empire, which had sided with Francis I against Charles V.[1][2][3] In 1500, Louis XII made an agreement with Ferdinand II on dividing the Kingdom of Naples, as Frederick IV was removed from the Neapolitan throne.

Foret and Francis I secured an alliance with the Ottoman Empire, giving France a strong army, ready to attack targets such as Marseille and Piedmont, areas close to the Italian province of Genoa.

Philippe de Chabot, a French general, led his army into Piedmont in March 1536, and proceeded to capture Turin the following month, but he failed to seize Milan.

In response, Charles invaded Provence, a region of France, advancing to Aix-en-Provence, and took Aix in August 1536 but his movement was halted by the French Army blocking routes to Marseilles.

While Charles V was busy fighting for territory in France, Francis I's armies received massive reinforcements in Piedmont in terms of generals, troops, and horses on a march headed for Genoa.

[5] A Franco-Turkish fleet was stationed in Marseille by the end of 1536, threatening Genoa, by planning to attack simultaneously with the French troops marching on land towards the city.

[6] The Truce of Nice, signed on June 18, 1538, ended the war, leaving Turin in French hands but affecting no significant change in the map of Italy.