Tsukushino Station (つくし野駅, Tsukushino-eki) is a passenger railway station located in the city of Machida, Tokyo, Japan, operated by the private railway operator Tokyu Corporation.

Located between Nagatsuta and Suzukakedai, it is 26.8 km from the terminus of the line at Shibuya.

[1] Only "Local" and "Semi-express" services stop at this station.

The station has two opposed side platforms serving two tracks, with an elevated station building located above and perpendicular to the platforms.

[1] In fiscal 2019, the station was used by an average of 11,544 passengers daily.