Tsurumi-Ono Station (鶴見小野駅, Tsurumi-Ono-eki) is a railway station operated by East Japan Railway Company (JR East) in Tsurumi-ku, Yokohama, Kanagawa Prefecture, Japan.

Tsurumi-Ono Station has two opposed side platforms serving two tracks, connected by a level crossing.

Tsurumi-Ono Station was opened on 8 December 1936 as Kōgyōgakkō-mae Stop (工業学校前停留場) on the privately held Tsurumi Rinkō Railway (鶴見臨港鉄道, Tsurumi Rinkō Tetsudō) and for passenger operations only.

The Tsurumi Rinkō line was nationalized on 1 July 1943 at which time the stop was elevated into status to that of a full station and given its present name.

Upon the privatization of the JNR on 1 April 1987 the station has been operated by JR East.