[1] The type collection was obtained from a mushroom market in Kunming, China (Yunnan Province), in 2010.

[1] Fruit bodies of Tuber microspermum are yellow-brown, roughly spherical, and measure up to 1.5 cm (0.6 in) in diameter.

The 80–120 μm-thick outer layer is made of somewhat angular to roughly spherical cells that are typically 7.5–12.5 μm in diameter.

The inner tissue layer of the peridium consists of interwoven hyaline (translucent), thin-walled hyphae measuring 2.5–5 μm wide.

Spores have a network pattern (reticulation) and discrete spines on their surface that are usually 4–6 μm long.