Turbidimetry

Turbidimetry (the name being derived from turbidity) is the process of measuring the loss of intensity of transmitted light due to the scattering effect of particles suspended in it.

[2] Turbidity-is an expression of optical look of a suspension caused by radiation to the scattered and absorbed wavelength.

A turbidometer measures the amount of radiation that passes through a fluid in forward direction, analogous to absorption spectrophotometry.

Standard for turbidimetry is prepared by dissolving 5g of hydrazinium (2+) sulfate(N2H4H2SO4) and 50g of hexamethylenetertramine in 1liter of distilled water is defined as 4000 nephelometric Turbidity Unit(NTU) Application Determination of water Clarity of pharma products and drinks Immunoassay in lab Turbidimetry offers little advantage than nephelometry in measurement of sensitivity in low level antigen a antibody immunoassay.

Antigen excess and matrix effects are limitations encountered Immunoturbidimetry is an important tool in the broad diagnostic field of clinical chemistry.