It passes through Fremont and heads to the northwest before turning to the south toward US 10 and WIS 49.

The highway then runs concurrently to the north with US 10 and WIS 49 for about five miles (8.0 km).

It runs concurrently to the north along both highways before WIS 54 splits off.

[3][a] This route, which would be the forerunner of WIS 110, started out as a 40-mile (64 km) long south-to-north US Highway located entirely within Wisconsin.

Due to a series of extensions and truncations in the 70 years since, however, WIS 110 is no longer designated on most of the former routing of US 110.