U.S. Route 169 (US-169) is a major north–south U.S. Highway that runs from US-64 in Tulsa, Oklahoma, to US-53 near Virginia, Minnesota.

A segment between Chanute and Iola is a freeway with fully controlled access with center concrete barrier, with two lanes in each direction.

From this point to the Missouri state line, US-169 alternates between freeways and surface streets.

It follows I-35 to Shawnee Mission Parkway in Overland Park, then travels east to Rainbow Boulevard.

The business route northern terminus is the junction of U.S. 169 & 6th Avenue in Garnett.