The third USS Josephine (SP-3295) was a United States Navy patrol vessel in commission from 1918 to 1919.

Josephine was built as a civilian motorboat of the same name in 1913 by Jacob Shipyard at City Island in the Bronx, New York.

The U.S. Navy acquired her from her owner, Frank L. Sample Sr., on 30 October 1918 for World War I service as a patrol vessel.

Assigned to the 3rd Naval District, Josephine served on the section patrol as a patrol and harbor craft in the New York City area.

World War I ended on 11 November 1918, twelve days after the Navy acquired Josephine, but she remained in service for a short time after the war before being decommissioned.