USS Rosalie was a captured Confederate sloop acquired by the Union Navy from the prize court during the American Civil War.

The sloop Rosalie, commanded by Master W. R. Postell, was intercepted and seized on 16 March 1863 by USS Octorara while attempting to run cargo from Bermuda into the besieged port of Charleston, South Carolina.

Sent to Key West, Florida, for adjudication, she was purchased by the Navy on 6 May 1863; fitted out as a tender; and commissioned in June 1863, Acting Ensign Charles P. Clark in command.

On 6 July she sighted her first large blockade runner, the schooner Ann accompanied by a small sloop; and a 3-day chase up Peace Creek ensued.

On 9 June 1864, Rosalie seized her only prize steamer, Emma, as that wood burner attempted to smuggle coal to Rebel forces.