The 2000–01 Ukrainian First League was the tenth season of the Ukrainian First League which was won by FC Dynamo-2 Kyiv.
The season started on July 23, 2000, and finished on June 28, 2001.
Three clubs promoted from the 1999-2000 Ukrainian Second League.
Three clubs were relegated from the 1999-00 Ukrainian Top League: In 2000-01 season, the Ukrainian First League consists of the following teams: Statistics are taken from here.
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