The Royal and Pontifical Higher University of San Francisco Xavier of Chuquisaca (USFX; Spanish: Universidad Mayor Real y Pontificia de San Francisco Xavier de Chuquisaca) is a public university in Sucre, Bolivia.

Founded in 1624 by order of the Spanish King Philip IV, and with the support of Pope Innocent XII, the university was intended to provide an education in Law and Theology to the families and descendants of the wealthy gentry of South America.

At the turn of the 19th century, Chuquisaca and its university came to constitute a center of revolutionary zeal in Bolivia.

The university intellectually sustained the well-cultivated Francophile elite whose ideals led to the Bolivian War of Independence and ultimately to the independence of all the Spanish colonies.

This Bolivian university, college, or tertiary institution related article is a stub.