[6] Fanat Kakhramonov (men's middleweight), 2019 world silver medalist Dilshodbek Ruzmetov (men's light heavyweight), Raykhona Kodirova (women's lightweight), and Maftunakhton Melieva (women's middleweight) completed the nation's boxing lineup by topping the list of eligible boxers from Asia and Oceania in their respective weight divisions of the IOC's Boxing Task Force Rankings.

Uzbekistan qualified a single boat in the women's C-2 500 m for the Games by finishing fifth in the final race at the 2019 ICF Canoe Sprint World Championships in Szeged, Hungary.

Malika Khakimova claimed a spot in the women's épée as one of the two highest-ranked fencers vying for qualification from Asia and Oceania in the FIE Adjusted Official Rankings.

Rasuljon Abdurakhimov and seven-time Olympian Oksana Chusovitina received a spare berth each in the men's and women's apparatus events, as one of the highest-ranked gymnasts, who were neither part of the team, nor qualified directly through the all-around, at the 2019 World Championships in Stuttgart, Germany.

Alexander Savkin and Alise Fakhrutdinova confirmed places each in the men's and women's event, respectively, with the former finishing fifth and the latter third among those eligible for Olympic qualification at the 2019 Asia & Oceania Championships in Kunming, China.

[12][13] Uzbekistan qualified one boat in the men's lightweight double sculls for the Games by winning the bronze medal and securing the second of three berths available at the 2021 FISA Asia & Oceania Olympic Qualification Regatta in Tokyo, Japan.

Rio 2016 Olympian Nikita Rafalovich qualified directly for the second time in the men's welterweight category (80 kg) by finishing among the top five Taekwondo practitioners at the end of the WT Olympic Rankings.