Vectorette PCR
[4] Multiple researches have taken this method as an opportunity to conduct experiments in order to uncover the potential uses that can be derived from Vectorette PCR.
[1] The first step includes utilizing a restriction enzyme in order to accomplish digestion of the sample DNA.
[11] The reason it is mismatched is to help it avoid Vectorette primers’ attempts at causing it to undergo first strand synthesis.
[11] This ligation brings together the vectorette which is double stranded and the ends of the restriction fragments which were previously made in the first step.
During the first part, the IP works on amplifying the primer extension while the VP remains hybridized with the product; thus, any background amplification is not carried out at this stage.
From this the scientists were able to show that the use of Vectorettes is capable of facilitating the recovery and mapping of sequences in complex genomes.
[5] Other work has looked at developing a method using Vectorette PCR in order to accomplish genomic walking.
By using Vectorette PCR, researchers have found a rapid technique to accomplish this with novel, microsatellite repeats.
Since Vectorette PCR is capable of being specific with its isolation and amplification of genes, this helped with their research and aided in improving the method of TE display by saving both time and costs.
[16] The researchers were then able to produce numerous dominant markers with the use of Vectorette PCR that is based on a TE display that is nonradioactive.
Vectorette PCR was used to uncover SSRs which flank the trinucleotide repeat that was targeted for testing.
They believe that their TNR method combined with the amplification provided by Vectorette PCR can be used in eukaryotes to create molecular markers that are based on simple repeat sequences.
For example, it gives rise to methods that can help during the outbreaks of diseases by making it easier to subtype pathogens that are similar or closely related.
[20] It is the region that gets cut out while exons are expressed, and so introns do not affect the code of amino acids.
[22] Vectorette PCR that utilizes the primers that originate from cDNA gives rise to a method that is capable of acquiring intron sequences which are located adjacent to exons and aiding in the development of the structure of genes.
These advantages allow the user to save time and resources while increasing the range of DNA that can be targeted.
Vectorette PCR comes in handy when it is necessary to obtain the regions that are both upstream and downstream and flank a sequence that is already known.
