He was the son of the Senator of the Kingdom of Italy, Ignazio Florio Sr. and baroness Giovanna D'Ondes Trigona.

He was the younger brother of Ignazio Florio Jr. Not inclined to business, he loved traveling and was a frequent visitor of Paris, Nice, Montecarlo and Cannes.

[2] His greatest fame came from creating the Targa Florio race in 1906, based on discussions with James Gordon Bennett Jr. and Henri Desgrange.

A magazine was initiated, Rapiditas, which aimed to enhance, with graphic and photographic reproductions of the race, the myth of the car and the typical character of modern life, speed.

In 1913 Vincenzo Florio founded the Automobile Club of Sicily, of which he remained president for a long time.