Known as St. Charles Road, the state highway runs 2.40 miles (3.86 km) from U.S. Route 421 (US 421) near Pennington Gap to SR 634 and SR 636 in St. Charles in northern Lee County.

SR 352 begins at an intersection with US 421 on the north side of Stone Mountain and west of Pennington Gap.

The state highway follows Straight Creek and Norfolk Southern's St. Charles Branch rail line north to the town of St. Charles.

Within the town, the state highway reaches its northern terminus at a three-way intersection with SR 634 (Bonny Blue Road) and SR 636 (Monarch Road).

[1][2] The entire route is in Lee County.