The route of the line including locations of significance was as follows:[1] Beginning in Calwa, a wye leads the Visalia District tracks east from the mainline and runs alongside Annadale road for some distance.

The line then curved southeast at DeWolf, served a packing house, and entered the town of Del Rey.

After crossing the Santa Fe Canal, the line ran alongside East Parlier Ave for a short distance, then between 1st and Fresno streets.

Leaving Reedley, the line continued to serve packing houses as it skirted the north end of Dinuba and crossed Road 80 / Alta Ave.

Entering Sultana, the line crossed CA J40 / Ave 416 at a steep angle as it served several large packing houses.

As the line curved due south to cross over CA-63, a diverging switch led east to a connection with the Porterville-Orosi District at Wyeth.

The rail line ran alongside the east side of CA-63 for several miles until it reached the large facility at Calgro, where the highway diverged southwest.

Entering Visalia, a small yard was present along with several spurs serving an ice house and other lineside industries.

Just after crossing CA-63 / Mooney Blvd, a spur ran north to serve a lumber mill and large winery.

Shortly, the line crossed the Southern Pacific's Bakersfield Subdivision, with an interlocking tower present.

[1] The line remained a valuable source of freight revenue for the Santa Fe through the 1970s, with produce being the predominant commodity.

[3] On May 12, 1992, the Santa Fe ran an Operation Lifesaver special from Calwa to Porterville and return, including a portion of the Visalia District.

EMD GP60M units 116 and 130 led the train, which was made up of a flat car carrying OLS signage and ATSF caboose # 999805.

[2] A truck body manufacturer in Tulare was served by the TVRR until early 1994, when a vehicle transloading facility was built in Exeter.

The TVRR itself became part of the San Joaquin Valley Railroad in 1998, including the small remaining sections of the Visalia District.

A short segment just northeast of Reedley at LacJac, approximately 1.2 miles, is still in use by the SJVR to serve a large wine and beverage-making industry.

[8] The short remaining track at Calwa (1.50 mi) is sometimes used to store ballast and Maintenance of way cars, and also serves lineside industries in the area.