After playing youth football for Náchod and Hradec Králové, he began his senior club career with the latter team.

[3] Born in 1973, Lokvenc started his professional career during the 1992–93 season with Hradec Králové,[4] joining Sparta Prague in October 1994.

[14] In the following round Kaiserslautern were paired with Slavia Prague; after the first match had finished goalless, Lokvenc scored the only goal in the second leg of their two-legged tie to eliminate the Czech team.

[16] Lokvenc played in the final of the competition at the end of May 2003, but his side were beaten 3–1 by league winners Bayern Munich as the latter claimed the double.

[20] Lokvenc signed for Red Bull Salzburg in the summer of 2005, rejecting offers from Portsmouth and Hertha Berlin.

[21] He described his move to Salzburg as "the best transfer of my life",[21] arriving around the same time as Germany national team players Thomas Linke and Alexander Zickler.

[23] He returned to the team in May 2006, taking part in a match for the first time since July 2005, as he made an appearance as a substitute in a 2–1 home loss against Pasching.

[27] In October 2007 Lokvenc scored the only goal in Salzburg's 1–0 UEFA Cup first round win against AEK Athens This was not enough for his team to advance, having lost 3–0 in the first leg of their two-legged tie.

[32] On 17 August 2008, the Czech played his first competitive match for FC Ingolstadt in a 3–2 victory against SpVgg Greuther Fürth on the first matchday of the second German Bundesliga.

[33] Lokvenc scored his first two goals on 5 October 2008 in a 4–2 win on the seventh matchday against Hansa Rostock (a relegated team from the Bundesliga).

After finishing his professional playing career, Lokvenc worked as a scout in the Czech Republic and Slovakia for his former team, Basel.

[35] He continued to be physically active after his football career, taking part in the 2010 Prague Half Marathon in the same field as former international teammate Pavel Nedvěd.

[36] He was again involved in the Prague Half Marathon in 2015, taking part in a relay alongside Nedvěd, fellow footballer Tomáš Hübschman and 2014 Miss Czech Republic, Tereza Skoumalová.

He took part as a substitute in the group stage match against United Arab Emirates and started the third place play-off game versus Uruguay, which the Czech Republic won to finish third overall in the tournament.

[38][39] At Euro 2000, Lokvenc made a substitute appearance in the Czech Republic's opening game against the Netherlands, replacing Pavel Nedvěd after 89 minutes as the match resulted in a 1–0 win for the Dutch.

[4] Lokvenc was particularly noted for his height, being referred to as a "towering forward",[55] and having "a similar aerial threat" to international teammate Jan Koller.