The 25-mile (40 km) highway begins at SR 22 in Mabton and travels north to Sunnyside, where it intersects Interstate 82 (I-82) and U.S. Route 12 (US 12).

SR 241 leaves Mabton after passing several suburban blocks and crosses over the Yakima River near a boat launch in the Sunnyside State Wildlife Recreation Area.

[5][6] The highway continues north through Givens Corner and across flat farmland predominately used for herb growing and vineyards.

It continues north into rolling farmland on Hanford Road, turning northeast to follow Sulphur Creek as it ascends into the Rattlesnake Hills.

[9] SR 241 turns north and northeasterly to follow a pass in the hills and begins its descent into the pastures of the Black Rock Valley.

[21] The three bridges carrying the highway over the Rosa Irrigation Canal system northeast of Sunnyside were rebuilt by the state government in 1977.

[25] The state government ultimately chose the Waneta interchange, but promised improvements to nearby roads to appease Mabton.