The Wayne County Regiment was a unit of the North Carolina militia that served during the American Revolution.

The North Carolina General Assembly created Wayne County and its regiment of militia out of part of Dobbs County and its regiment of militia on November 2, 1779.

[1] The Wayne County Regiment was part of the New Bern District Brigade of the North Carolina militia, commanded by Brigadier General Richard Caswell.

Both John Sheppard and Benjamin Exum served as colonels and commanders jointly of the Wayne County Regiment.

After the war, John Sheppard was a representative in the North Carolina state legislature in 1784–1785.