The Whissendine Brook is a small watercourse in the East Midlands region of England.

The Whissendine Brook rises to the west of Cold Overton in Leicestershire and flows in a north easterly direction past the village and across the county boundary into Rutland.

From here it continues through a gentle valley, splitting the village of Whissendine into two distinct halves, with the older settlement on higher ground to the east and a larger collection of more modern houses around a restored 19th century windmill to the west.

Upon leaving the village, it continues to flow north through arable fields where it soon joins the Langham Brook back on the border of Leicestershire.

In 2016, the overall classification of the Whissendine Brook was 'Poor' under the Water Framework Directive for reasons of sewage contamination, poor livestock management and poor nutrient management in the agricultural land surrounding it.