The Rothley Brook is a left bank tributary of the River Soar in Leicestershire, England.

It then flows through Desford and Ratby before being joined by a tributary from Kirby Muxloe just before Glenfield.

After Glenfield, it flows around Anstey, Cropston and Thurcaston before finally reaching Rothley where it joins the River Soar.

[1] The Rothley Brook is one of the defining features of the large Castle Hill Country Park in Anstey and runs parallel to Cropston Road and the A46 as well as passing underneath Anstey Lane, Thurcaston.

The parts of the brook which flow through Castle Hill Park are a haven for wildlife, including kingfishers.