In 1521, after having studied theology in Freiburg and Tübingen, Reublin became the pastor at St. Alban in Basel and began to advocate reform.
In the Fall of 1522 Reublin was expelled from the city for his Reformation sermons and moved to Witikon in 1524, where he became the local pastor and preached against infant baptism.
Reublin was with Conrad Grebel and Felix Manz in Zürich in January 1525 at the birth of the Anabaptist movement.
After an arrest in Strasbourg in the Winter of 1528–29, Reublin wandered with other Swiss Brethren to Auspitz in Moravia where the first farms of the Hutterites were established.
After a dispute over the sharing of personal resources and money, Reublin was banned from the local community and returned to southwest Germany in 1531, where he was again evangelizing in Rottenburg.