[2] On 14 February 1553-4 Ayloffe was admitted a student of Lincoln's Inn, where two other near relatives, bearing the same name, distinguished themselves in the sixteenth century, and in 1560 he was called to the bar.
After being appointed "reader" at his inn of court in Lent term, 1571, he was made serjeant-at-law in 1577, at the same time as Sir Edmund Anderson, afterwards the well-known lord chief justice of the Common Pleas.
A notice of a banquet in the Middle Temple hall, given by Ayloffe with other barristers upon whom a similar distinction had just been conferred, to celebrate their promotion, is preserved among the Ashmolean manuscripts at Oxford.
He was present in 1581 at the trial of Edmund Campion and other seminary priests, and special attention is called to the part he played on that occasion in a pamphlet published by English Catholics at Pans shortly afterwards, and bearing the title An Epistle of Comfort to the Reverend Priestes and to the Honorable, Worshipful and other of the Laye sort restrayned in Durance for the Catholike Fayth, 12mo.
Some letters that passed between Ayloffe and the lord mayor of London with reference to the appointment of his brother as town clerk, are preserved among the city archives for the years 1580 and 1581.