William Clarke Chambers (December 17, 1862 – February 1, 1958) was a Canadian railway contractor, farmer and politician in Ontario.

He represented Wellington West in the Legislative Assembly of Ontario from 1911 to 1919 and from 1924 to 1926 as a Conservative.

[1][2] The son of Andrew Chambers and Lucy Clarke, he was born in Wroxeter and grew up on the family farm.

He was educated at public schools in Blanchard and Harriston.

[1] In 1891, he married Jennie Viola McAndless; the couple had three sons and six daughters.