The highway is located in northwestern Big Horn County and travels from the unincorporated community of Emblem north along the Park-Big Horn county line north to the town of Byron and east to Lovell.

WYO 32 continues north, paralleling the county line for a distance, but then turns northeast, roughly paralleling the Shoshone River which runs to the north.

[1] WYO 32 south of Byron to Lovell travels southwest to northeast, intersects Wyoming Highway 33 south of Lovell before turning due north and ending at US 14A / US 310 / WYO 789.

Mileposts increase from north to south along Wyoming 32.

The entire route is in Big Horn County.