Wyoming Highway 353 (WYO 353) is a 15.51-mile-long (24.96 km) east-west Wyoming state road located in east-central Sublette County.

WYO 353 provides travel between U.S. Route 191 (US 191) at Boulder to Big Sandy and eastern Sublette County.

Wyoming Highway 353 begins its western end at U.S. Route 191 in Boulder.

Highway 353 travels eastward from Boulder, and meets the East Fork River which it roughly parallels it for most of its length.

WYO 353 turns southeasterly and will remain on that routing till its end.