The first full-production set, 10031, was delivered in July 2007, being hauled via JR tracks from the Kawasaki factory in Hyōgo Prefecture.

The trains entered service from 30 March 2008 with the opening of the Green Line.

[3] Two second-batch four-car sets were introduced from the start of the revised timetable on 29 March 2014.

[5] In 2018, it was announced that ten of the 17 sets in operation would be lengthened to six cars each by 2024 as a measure to ease overcrowding on the Green Line.

From 25 February 2018, set 10161 began operating in a special cream and green vinyl wrapping livery based on the livery carried by former Yokohama city tramcars to mark the tenth anniversary of the opening of the Green Line.