The brigade is participating in the defense against the Russian invasion of Ukraine in the country's east.

[1] On 16 October 2018, as part of a working trip to Lviv Oblast, the then President, Petro Poroshenko took part in several events dedicated to the Day of Territorial Defense Soldiers, which took place at the International Center for Peacekeeping and Security.

[2] From 17 to 20 July 2019, about two hundred reserve officers of the brigade underwent combat training at the International Center for Peacekeeping and Security.

[4][irrelevant citation] On 30 September, Ukrainian president Zelensky commended the 103rd Brigade, announcing that it was responsible for the liberation of Yampil in the Donetsk Oblast, along with the 214th Battalion of the Special Operations Forces.

[6] In September and October 2024, it was reported that the 103rd Brigade was participating in Ukrainian operations in Russia's Kursk Oblast.