The 1940–41 Divizia B was the seventh season of the second tier of the Romanian football league system.
Feroemail Ploiești was excluded, being another victim of the regime's law of banning workers' teams.
Maccabi București, sport club, representing the Jewish community was expelled from all the official competitions by the same legionary regime, which adopted antisemitic policies.
CA Oradea, Crișana Oradea, CS Târgu Mureș, Mureșul Târgu Mureș, Olimpia CFR Satu Mare, Oltul Sfântu Gheorghe, Stăruința Oradea, Victoria Carei and Victoria Cluj moved in the Hungarian football league system due to the Second Vienna Award which was signed on 30 August 1940, territory of Northern Transylvania being assigned from Romania to Hungary.
Dragoș Vodă Cernăuți and Muncitorul Cernăuți (Northern Bukovina), Maccabi Chișinău, Nistru Chișinău and Traian Tighina (Bessarabia) were not allowed to play anymore in the Romanian football league system due to the Soviet occupation of Bessarabia and Northern Bukovina during June 28 – July 4, 1940, which had as result the Soviet annexation of the region.