The 1st Florida Cavalry Regiment was a Confederate army unit during the U.S. Civil War, originally organized in July 1861 at Tallahassee.

Members of the regiment came primarily from Alachua, Clay, Columbia, Duval, Leon, Levy, Nassau and Suwannee counties.

[1] William G. M. Davis, a relatively wealthy lawyer from Leon County, used his own funds to start the regiment and was elected colonel of the unit.

Companies that made up the unit were raised from the following counties: Alachua, Clay, Columbia, Duval, Leon, Levy, Nassau, and Suwannee.

[4] The 1st Florida Cavalry Regiment surrendered in North Carolina in April 1865.