The 2000 NCAA Division I lacrosse tournament was the 30th annual tournament hosted by the National Collegiate Athletic Association to determine the team champion of men's college lacrosse among its Division I programs, held at the end of the 2000 NCAA Division I men's lacrosse season.

[1] This marked the seventh victory in a national championship game for the Syracuse program.

[a] The championship game was played at Byrd Stadium at the University of Maryland in College Park, Maryland, with 24,105 fans in attendance.

[3] Twelve NCAA Division I college men's lacrosse teams met after having played their way through a regular season, and for some, a conference tournament.

No teams made their debut appearance in the Division I lacrosse tournament.