2001 Copa Colsanitas – Singles

Patricia Wartusch was the defending champion but lost in the second round to Mariana Díaz Oliva.

Paola Suárez won in the final 6–4, 6–2 against Rita Kuti-Kis.

A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated.

The top two seeds received a bye to the second round.