The 2005–06 FA Trophy was the thirty-sixth season of the FA Trophy.
Grays Athletic defended their trophy, only the fourth time this has been achieved in the competition's history.
The teams from Conference North and Conference South entered in this round.
The teams from Conference National entered in this round.
Man of the Match: Michael Kightly Assistant referees: Gavin Ward (Kent) Trevor Massey (Manchester) Fourth official: Keith Stroud (Hampshire)