During the 2016–17 season, AFC Ajax participated in the Eredivisie, the KNVB Cup, the UEFA Champions League and the UEFA Europa League.The first training took place on 25 June 2016.

The traditional AFC Ajax Open Day was held on 29 July 2016.

Appearances for competitive matches only As of 24 May 2017 Eredivisie KNVB Cup UEFA Champions League UEFA Europa League All times are in CEST

For a list of all Dutch football transfers in the summer window (1 July 2016 to 31 August 2016) please see List of Dutch football transfers summer 2016.

For a list of all Dutch football transfers in the winter window (1 January 2017 to 1 February 2017) please see List of Dutch football transfers winter 2016–17.