This is a list of players who graduated from the Challenge Tour in 2016.
* European Tour rookie in 2017 T = Tied The player retained his European Tour card for 2018 (finished inside the top 101 or the top 10 of the Access List).
The player did not retain his European Tour card for 2018 (finished outside the top 147).
Ritthammer won three times on the Challenge Tour in 2016.
A change before the season to the tour regulations allowed amateurs to earn ranking points, while also dictating that any player within the top 15 who earned points as an amateur would be counted in addition to the usual 15 graduates; therefore, since Langasque had earned 24,200 points as an amateur, the number of graduates was increased to 16.