The 2016 Iowa State Cyclones football team represented Iowa State University in the 2016 NCAA Division I FBS football season.

They were led by first-year head coach Matt Campbell.

Iowa State announced their 2016 football schedule on November 19, 2015.

The 2016 schedule comprised 7 home and 5 away games in the regular season.

The Cyclones hosted Big 12 foes Baylor, Kansas State, Oklahoma, Texas Tech, and West Virginia and traveled to TCU, Oklahoma State, Texas, and Kansas.