The 2019–20 Southern Miss Lady Eagles basketball team represented the University of Southern Mississippi during the 2019–20 NCAA Division I women's basketball season.

The Lady Eagles, led by sixteenth-year head coach Joye Lee-McNelis, played their home games at Reed Green Coliseum and were members of Conference USA (C-USA).

They finished the season 15–15, 7–11 in C-USA play, to finish in a tie for ninth place.

They lost in the first round of the C-USA women's tournament to Marshall.

The day following their loss, the rest of the games for the postseason tournament were cancelled due to COVID-19 concerns.