If still tied, extra time was not played, and a penalty shoot-out was used to determine the winner (Regulations Article 2.4.2).
Tied 2–2 on aggregate, Unión won on away goals and advanced to the round of 16 (Match B).
Tied 1–1 on aggregate, Unión La Calera won on away goals and advanced to the round of 16 (Match C).
Tied 3–3 on aggregate, Deportivo Cali won on penalties and advanced to the round of 16 (Match E).
Tied 6–6 on aggregate, Lanús won on away goals and advanced to the round of 16 (Match H).
Tied 1–1 on aggregate, Vélez Sarsfield won on away goals and advanced to the round of 16 (Match E).