[2] The draw for the fourth round was held on 1 September 2020, 11:30 at CBF headquarters in Rio de Janeiro.
[3] The 10 qualified teams were drawn in a single group (CBF ranking shown in parentheses).
In the fourth round, each tie was played on a home-and-away two-legged basis.
If the aggregate score was level, the second-leg match would go straight to the penalty shoot-out to determine the winner.
América Mineiro won 5–3 on aggregate and advanced to the round of 16.