The 2020 TK Sparta Prague Open was a women's tennis tournament played on Clay in Prague, Czech Republic, and was part of the 2020 WTA 125K series.

This was the first edition and was held from 29 August to 6 September 2020.

Kristína Kučová won the title, defeating Elisabetta Cocciaretto in the final, 6–4, 6–3.

[1] Click on the seed number of a player to go to their draw section.